On-chip Nanomanipulation of Single Influenza Virus Using Dielectrophoretic Concentration and Optical Tweezers
نویسندگان
چکیده
We developed manipulation of single virus using optical tweezers and dielectrophoretic (DEP) force in a microfluidic chip. We used a microfluidic chip made of poly(dimethylsiloxane) (PDMS). The chip has independent virus chamber and cell chamber to make quantitative analysis of the functions of influenza virus before and after infection to a cell. DEP force integrated inside virus chamber concentrates the virus. Concentrated viruses flow to the part for sample selection. The selected virus was transported to the cell chamber using optical tweezers. After virus transport, these chambers were isolated by photocrosslinkable resin. In this paper, we demonstrated DEP concentration of the influenza virus, manipulation of the single virus to transport the cell chamber, contact to a target cell and separation of chambers in the chip.
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